- #IMAGEJ SOFTWARE CELL STAINING FOR FREE#
- #IMAGEJ SOFTWARE CELL STAINING MAC OS#
- #IMAGEJ SOFTWARE CELL STAINING FREE#
It occurs during development and regulation of tissue homeostasis or as a result of changes in environmental stimuli. The virtual slide(s) for this article can be found here:Īpoptosis is an active process of self-destruction, whereby cells undergo physiological cell death. ImageJ 1.43 n may therefore provide a useful tool for the assessment and discrimination of apoptotic cells. Consequently, a quantitative measure of apoptosis can be obtained that is linked to morphological changes.
#IMAGEJ SOFTWARE CELL STAINING FREE#
Nuclear area factor can be calculated using powerful free and open-source software. NAF was the most reliable parameter in assessment of apoptosis. Image analysis results correlated positively and significantly with the results obtained by cell viability assay (R = 0.958, P-value = 0.042). This decrease was assumed to indicate DNA loss. Some of the other studied parameters showed also the same trend.
Image analysis revealed a progressive and highly significant decrease in nuclear area factor detected in apoptotic cells and in cells suspected of undergoing apoptosis compared to the control cells (P-values < 0.01). Cell viability assay and nuclear image analysis of stained Hep2 cells were used to discriminate apoptotic cells and cells suspected to be undergoing apoptosis from control cells based on parameters such as nuclear area, circularity, perimeter and nuclear area factor (NAF), in association with visual morphology. Study DesignĬisplatin in different concentrations was used to induce apoptosis in cultured Hep2 cells. Some may prefer popular software such as Adobe Photoshop, which is the world’s best imaging and design app.To verify the efficacy of ImageJ 1.43 n in determining the extent of apoptosis which is a complex and multistep process. To put it simply, this software is not everyone’s cup of tea. Although, you’ll have to spend your time studying some of its crucial features since it’s designed for a specific audience. ImageJ is simply the best image-processing freeware for scientific purposes.
#IMAGEJ SOFTWARE CELL STAINING FOR FREE#
You can access the download link for free on the software’s official website.
#IMAGEJ SOFTWARE CELL STAINING MAC OS#
Since the software is written in Java, you can run it on Windows, Mac OS X, and Linux in both 32-bit and 64-bit modes. While its nature as an online applet allows you to develop your own mini-programs. You can also create and debug macros to automate the tasks you used most often. The software also allows you to download or write additional plug-ins to customize the program to fit your research needs. You can also make your own image calculations using the program and create statistics according to your preferred parameters such as mean and standard deviations. It is simply interactive which allows you to easily edit and analyze images for printing. Basic functions of the toolbar includes a wide range of measurement tools, image selection and enhancement, geometric operations, scaling and color processing. The software’s mini structure allows you to display and process multiple images at the same time. Once you’ve downloaded the program, you’ll get a mini toolbar which gives you access to the program’s main functions including the menu bar and status/progress bar. The software is also a public domain, which means you’re allowed to add plug-ins and make changes to its program and share it to the user community without any copyright restriction. It was created and constantly revamped by US software developer Wayne Rasband at the National Institutes of Health. ImageJ is an open-source freeware designed for scientific image processing and analysis. After its release, the software became a significant aid for everyday scientific researches and discoveries.
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